Fig. 3
From: A new enzyme-linked immunosorbent assay (ELISA) for human free and bound kallikrein 9
Size exclusion chromatographic separation of a tonsil tissue extract and b ovarian cancer ascites. Samples were fractionated and the collected fractions were analyzed by ELISA using the following antibody pairs: a mouse mAbs 28ED436 and 4ED28.2; b mouse mAb 28ED436 and rabbit pAb anti-serpinA3. The corresponding molecular mass of each peak was estimated according to molecular mass standards, eluting as indicated by arrows on top panel. In tonsil, the major form is free KLK9, eluting at a molecular mass of ~35 kDa. In ascites, the major form is KLK9 bound to a1-antichymotrypsin (KLK9-ACT), eluting around 100 kDa. For more discussion see text