Fig. 1

Representative two-dimensional protein gel of CNE2 cell lysate proteins with accompanying western blots. a CNE2 cell lysate proteins were separated by two-dimensional PAGE, transferred to PVDF membrane, and then incubated with diluted sera (1:250) from a patient with NPC. b PVDF membrane incubated with sera from another patient with NPC. c PVDF membrane incubated with sera from a normal control. PVDF membranes were incubated with appropriate secondary antibodies and visualized by chemiluminescence. d Silver stained two-dimensional gel for total protein isolated from the CNE2 NPC cell line