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Fig. 1 | Clinical Proteomics

Fig. 1

From: OLFM4, KNG1 and Sec24C identified by proteomics and immunohistochemistry as potential markers of early colorectal cancer stages

Fig. 1

Proteomic discovery experiment workflow. Tissue section manual macrodissections (see line surrounding specific tissue area selected) were performed to obtain an enriched population of epithelial cells. The FASP-FFPE™ protein digestion kit was applied on each patient tissue (using around 300 mm2 of 6 µm tissue section). FASP-FFPE™ was applied on the dry material resuspended in the UPX buffer. Four to five patient sample digests were grouped per pool analysed. Four pools per disease groups were analysed by label-free proteomics differential analysis. UPX universal protein extraction

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