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Table 1 Glycated peptides of different plasma proteins quantified in tryptic digests of type 2 diabetes and control plasma samples

From: Glycated lysine-141 in haptoglobin improves the diagnostic accuracy for type 2 diabetes mellitus in combination with glycated hemoglobin HbA1c and fasting plasma glucose

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Sequence

Protein symbol (Accession number, glycation site)

t R (min)

Q1 m/z (±0.2)

Q3 m/z (±0.2)

1

TCVADESAENCDKSLHTLFGDK

HSA (P02768; K64)

13.8

887.1

869.1

2

SLHTLFGDKLCTVATLR

HSA (P02768, K73)

17.9

698.7

680.7

3

ETYGEMADCCAKQEPER

HSA (P02768, K93)

8.8

746.0

136.1

4

ETYGEMADCCAKQEPER

HSA (P02768, K93)

7.9

751.3

136.1

5

AAFTECCQAADKAACLLPK

HSA (P02768, K174)

15.9

763.0

120.0

6

AACLLPKLDELRDEGK

HSA (P02768, K181)

16.2

664.0

646.0

7

AEFAEVSKLVTDLTK

HSA (P02768, K233)

18.6

907.0

880.0

8

ADLAKYICENQDSISSK

HSA (P02768, K262)

13.6

1052.5

1025.5

9

TYETTLEKCCAAADPHECYAK

HSA (P02768, K359)

9.9

670.8

237.1

10

VFDEFKPLVEEPQNLIK

HSA (P02768, K378)

18.6

1104.1

1077.1

11

KVPQVSTPTLVEVSR

HSA (P02768, K414)

14.9

601.3

900.5

12

KQTALVELVK

HSA (P02768, K525)

13.1

645.9

603.9

13

EQLKAVMDDFAAFVEK

HSA (P02768, K545)

18.7

668.3

120.1

14

KLVAASQAALGL

HSA (P02768, K574)

16.3

652.4

501.8

15

VQWKVDNALQSGNSQESVTEQDSK

IGKC (P01834, K41)

12.7

947.1

941.1

16

DSTYSLSSTLTLSKADYEK

IGKC (P01834, K75)

16.4

757.7

751.7

17

VYACEVTHQGLSSPVTKSFNR

IGKC (P01834, K99)

12.6

848.1

830.1

18

QVKDNENVVNEYSSELEK

FGB (P02675, K163)

12.8

762.7

72.1

19

SKAIGYLNTGYQR

A2M (P01023, K1003)

10.5

816.9

1255.6

20

ALLAYAFALAGNQDKR

A2M (P01023, K1162)

18.5

628.7

86.1

21

KCSTSSLLEACTFR

TF (P02787, K683)

13.8

911.4

884.4

22

ADSSPVKAGVETTTPSK

IGLC (P01842, K50)

8.7

613.0

70.1

23

AKVQPYLDDFQK

APOA1 (P02647, K120)

12.5

807.4

765.4

24

KWQEEMELYR

APOA1 (P02647, K131)

10.1

530.6

524.6

25

AVGDKLPECEAVCGKPK

HP (P00738, K141)

9.4

674.0

656.0

26

M KGLIDEVNQDFTNR

FGA (P02671, K71)

14.9

653.3

894.4

27

SSSYSKQFTSSTSYNR

FGA (P02671, K581)

8.7

664.6

658.6

  1. The timed multiple reaction monitoring relied on the retention time of each peptide in RP-HPLC and a specific precursor/fragment ion pair (Q1/Q3 mass range)
  2. t R retention time, C, M , and K carbamidomethylated cysteine, methionine sulfoxide, and fructosamine lysine, HSA human serum albumin, IGKC Ig kappa chain c region, FGB fibrinogen beta chain, A2M alpha-2-macroglobulin, TF serotransferrin, IGLC Ig lambda chain C region, APOA1 apolipoprotein A-I precursor, HP haptoglobin, FGA fibrinogen alpha chain