Fig. 1

ATP1A1downregulation renal cell carcinoma. A One representative pair of isotope peptides of ATP1A1 with the sequence “SPFTENPLETR” (m/z 760.38/761.88, 2+) was used to quantify its differential expression level between human RCTs and PKTs. The mass spectra of this peptide from the protein mixture of PKTs and labeling HEK293 cells (a). The mass spectra of this peptide from sample mixture of RCTs and labeling HEK293 cells (b). The β-actin was taken as a loading control. B Analysis of relative expression of ATP1A1 in renal cell carcinoma tissues (RCTs) and autologous para-cancerous kidney tissues (PKTs) by Western blot