Fig. 4

ATP1A1 overexpression inhibits cell proliferation and cell migration of RCC cells. a, b Expression level of ATP1A1 was determined by Western blot in RCC cells transfected with pYR-ATP1A1 plasmids (ATP1A1) or the empty pYR vector (Control). Mock: RCC cells without transfection. c, d Cell proliferation was detected at transfection for 0, 24, 48, 72 h in RCC cells. The experiment was performed in triplicate. *P < 0.05. e, f Cell migration of OS-RC-2 and 786-0 cells transfected with pYR-ATP1A1 plasmids (ATP1A1) or the empty vector (Control) by a transwell assay. Scale bar 100 μm (original magnification ×200). The experiment was performed in triplicate, and data were shown as mean ± SD, *P < 0.05