Downregulation of ATP1A1 promotes cancer development in renal cell carcinoma

Table 1 Quantitative identification of ATP1A1 expression in renal carcinoma

Protein	MOWSE scores (queries match)		No. of Leu-containing peptide^c		SILAC-ratio^d (mean ± SD)		Change ratio^e (ratio 1/ratio 2)
Protein	RCT^a	PKT^b	RCT^a	PKT^b	Ratio 1	Ratio 2	Change ratio^e (ratio 1/ratio 2)
ATP1A1	283 (15)	817 (38)	1	1	0.45	1.678	0.27
β-actin	380 (30)	288 (23)	5	5	1.01 ± 0.09	0.95 ± 0.08	1.06

^aThe protein mixture from RCT and Leu-d₃ labeling HEK293 cells; RCT renal carcinoma tissue
^bThe protein mixture from PKT and Leu-d₃ labeling HEK293 cells; PKT para-cancerous kidney tissues
^cThe number of unique Leu-containing isotope peptides used for MS quantification
^dAverage SILAC-ratio, from the intensity between tissues versus labeling HEK293 cells, was quantified using Analyst QS software. SILAC ratio 2: RCT/HEK293; SILAC ratio 1: PKT/HEK293
^eThe change ratio between two tissues (SILAC ratio 1/SILAC ratio 2)

ISSN: 1559-0275