Fig. 7

Schematic diagram of lung tissue specimens using for proteomic analysis. The dissected tissues in lysis buffer were sonicated to extract proteins. After 4-plex iTRAQ labeling, 10% of the peptides were analyzed for global proteins and 90% of the peptides were used for enrichment of glycosite-containing peptides by Solid-phase extraction of N-linked glycopeptides (SPEG)