Table 4 Putative proteins shared among bPPD, aPPD and P22 that may contribute to cross-reactivity
No. | NCBI accession | Description | Relative abundance in % Mean ± SD | AAP | Ident/query coverage | |||
|---|---|---|---|---|---|---|---|---|
bPPD | aPPD | P22 | bPPD | aPPD | ||||
1 | 489513185 4566238 | Molecular chaperone GroES 10 kDa heat shock protein | 3.6 ± 0.0 | 1.1 ± 0.1 | 1.0 ± 0.3 | 1.0243 | 1.0261 | 97/100 |
2 | 489497323 497665169 | Molecular chaperone GroEL Molecular chaperone GroEL | 3.0 ± 0.2 | 1.6 ± 0.2 | 2.5 ± 0.6 | 1.0251 | 1.0244 | 94/100 |
3 | 489516779 489971186 | ESAT-6-like protein EsxL Hypothetical protein | 2.2 ± 0.1 | 0.7 ± 0.2 | 1.4 ± 0.3 | 1.0124 | 1.0238 | 87/100 |
4 | 489503953 489971186 | ESAT-6-like protein EsxN Hypothetical protein | 2.1 ± 0.0 | 0.7 ± 0.2 | 1.2 ± 0.4 | 1.0193 | 1.0238 | 94/100 |
5 | 489496900 497665296 | Molecular chaperone DnaK Molecular chaperone DnaK | 1.5 ± 0.1 | 0.8 ± 0.2 | 0.9 ± 0.1 | 1.0157 | 1.0149 | 95/94 |
6 | 489500428 489971186 | ESAT-6-like protein EsxI Hypothetical protein | 1.4 ± 0.1 | 0.7 ± 0.2 | – | 1.0154 | 1.0238 | 87/100 |
7 | 489498552 489970303 | Elongation factor Tu Elongation factor Tu | 1.2 ± 0.0 | 1.3 ± 0.4 | 2.0 ± 0.2 | 1.0225 | 1.0226 | 97/100 |
8 | 489506691 489969124 | Meromycolate extension acyl carrier protein Acyl carrier protein | 1.0 ± 0.1 | 0.7 ± 0.3 | – | 1.0236 | 1.0243 | 92/100 |
9 | 499253161 489971186 | Secretion protein Hypothetical protein | 0.7 ± 0.2 | 0.7 ± 0.2 | – | 1.0113 | 1.0238 | 93/100 |
10 | 489506256 489968974 | Cell wall synthesis protein Wag31 DivIVA domain-containing protein | 0.7 ± 0.1 | 0.7 ± 0.2 | 1.0 ± 0.4 | 0.9988 | 1.0052 | 86/100 |
11 | 489501012 497661234 | 5-Methyltetrahydropteroyltriglutamate 5-Methyltetrahydropteroyltriglutamate | 0.6 ± 0.0 | 0.1 ± 0.0 | 1.8 ± 0.5 | 1.0388 | 1.0374 | 86/99 |
12 | 489500440 489971185 | peptidase M22 peptidase M22 | 0.5 ± 0.0 | 0.7 ± 0.3 | 0.6 ± 0.2 | 0.9816 | 0.9844 | 87/100 |
13 | 489498442 489970321 | 50S ribosomal protein L7/L12 50S ribosomal protein L7/L12 | 0.5 ± 0.0 | 1.4 ± 0.0 | – | 1.0392 | 1.0327 | 93/100 |
14 | 489504514 489973362 | Bacterioferritin Bacterioferritin | 0.5 ± 0.1 | 2.7 ± 1.6 | 0.8 ± 0.1 | 1.0193 | 1.0226 | 88/100 |
15 | 489495992 497665638 | Diacylglycerol acyltransferase Diacylglycerol acyltransferase | 0.5 ± 0.1 | 0.6 ± 0.0 | 0.9 ± 0.3 | 1.0142 | 1.0141 | 85/95 |
16 | 489504572 500042691 | Diacylglycerol acyltransferase Diacylglycerol acyltransferase | 0.5 ± 0.01 | 0.9 ± 0.1 | 1.0 ± 0.1 | 1.0166 | 1.0192 | 81/95 |
17 | 489998054 656316315 | Serine protease Peptidase S1 | 0.5 ± 0.0 | 0.9 ± 0.2 | – | 1.0357 | 1.0264 | 73/96 |
18 | 489503193 497663153 | Universal stress protein Universal stress protein | 0.4 ± 0.0 | 0.6 ± 0.0 | 0.4 ± 0.0 | 1.0434 | 1.0431 | 88/100 |
19 | 489513178 497664719 | Molecular chaperone GroEL Molecular chaperone GroEL | 0.4 ± 0.0 | 0.7 ± 0.1 | 0.6 ± 0.1 | 1.0416 | 1.0399 | 84/98 |
No. | NCBI accession | Description | Relative abundance (%) ± SD | AAP | Ident/query coverage | |||
|---|---|---|---|---|---|---|---|---|
aPPD | P22 | bPPD | aPPD | |||||
20 | 554796584 656316442 | aconitate hydratase aconitate hydratase | 0.3 ± 1.0 | 0.6 ± 0.2 | 1.0 ± 0.2 | 1.0252 | 1.0245 | 89/99 |
21 | 489509166 497663814 | Hypothetical protein Hypothetical protein | 0.3 ± 0.0 | 0.7 ± 0.2 | – | 1.0146 | 1.0152 | 87/100 |
22 | 489997754 500042691 | diacylglycerol acyltransferase diacylglycerol acyltransferase | 0.2 ± 0.1 | 0.9 ± 0.1 | 1.0 ± 0.1 | 1.0225 | 1.0188 | 83/96 |
23 | 489501803 497661528 | ATP synthase subunit beta F0F1 ATP synthase subunit beta | 0.2 ± 0.0 | 0.6 ± 0.1 | 0.3 ± 0.3 | 1.0239 | 1.0238 | 95/98 |
24 | 489496308 564987547 | Acyl dehydratase Acyl dehydratase | 0.1 ± 0.1 | 1.4 ± 0.7 | 0.7 ± 0.0 | 1.0414 | 1.0457 | 80/99 |
25 | 489509098 489972034 | Iron-dependent repressor IdeR Dihydrofolate reductase | 0.1 ± 0.0 | 0.2 ± 0.0 | – | 1.0362 | 1.0409 | 93/100 |
26 | 489996564 497664056 | D-3-phosphoglycerate dehydrogenase Phosphoglycerate dehydrogenase | 0.1 ± 0.0 | 0.3 ± 0.0 | – | 1.0603 | 1.0551 | 89/100 |
27 | 489500222 495529442 | Molybdenum cofactor biosynthesis protein Molybdenum cofactor biosynthesis protein | 0.1 ± 0.0 | 0.1 ± 0.0 | 0.3 ± 0.4 | 1.0536 | 1.0476 | 96/91 |
28 | 554793963 497660570 | Hypothetical protein O217_19000 Hypothetical protein | 0.1 ± 0.0 | 0.4 ± 0.0 | 0.2 ± 0.2 | 1.0490 | 1.0422 | 85/89 |
29 | 489497388 489970521 | Dihydrolipoyl dehydrogenase Dihydrolipoyl dehydrogenase | 0.1 ± 0.0 | 0.1 ± 0.0 | 0.3 ± 0.3 | 1.0408 | 1.0343 | 90/99 |
30 | 489497373 489970531 | hypothetical protein hypothetical protein | 0.1 ± 0.0 | 1.2 ± 0.2 | – | 1.0212 | 1.0135 | 76/100 |
31 | 489997334 497663425 | Phosphoglycerate kinase Phosphoglycerate kinase | 0.1 ± 0.0 | 0.2 ± 0.0 | – | 1.0415 | 1.0428 | 82/88 |
32 | 489497373 489970531 | Hypothetical protein Hypothetical protein | 0.1 ± 0.0 | 1.2 ± 0.2 | – | 1.0209 | 1.0136 | 76/100 |