Fig. 1

Efficiency of protein to peptide conversion after digestion with LysC, trypsin and chymotrypsin. The yields were expressed as a ratio of the amount of eluted peptides to total protein amount processed (a). The number of unique peptides identified after sequential digestion with LysC (L), LysC + trypsin (L + T), LysC + trypsin + chymotrypsin (L + T + CT) or peptide fractionation by pipet-tip strong anion exchange (SAX) protocol (b). The number of proteins identified after sequential digestion with L, L + T, L + T + CT or peptide fractionation by SAX (c). Sequence coverage obtained after sequential digestion with L, L + T, L + T + CT or peptide fractionation by SAX for three different proteins: fibrinogen gamma chain, coagulation factor XIII a and antithrombin-III (d). *p < 0.05 as compared to L, ^# p < 0.05 as compared to L + T, ^† p < 0.05 as compared to L + T + CT; mean ± SEM; n = 4 per group