Fig. 2From: Random and independent sampling of endogenous tryptic peptides from normal human EDTA plasma by liquid chromatography micro electrospray ionization and tandem mass spectrometryTotal protein analysis by the Dumbroff method of non-covalent binding to filter paper followed by washing of non-proteinaceous components with methanol prior to staining with 0.1 g CBBR250 in 40% methanol and 10% acetic acid. Plasma samples were mixed 1:1 with 2 × tris sample buffer for SDS-PAGE and then diluted a further 10 fold in 2 × tris sample buffer (dF 20) into the linear BSA standard rangeBack to article page