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Table 1 The summary of the total number of peptide correlations and the number of protein accession numbers detected from a total of 563,095 MS/MS spectra from human EDTA plasma from the sum of MASCOT, OMSSA, X!TANDEM and SEQUEST against a library of 158,072 human protein accessions with respect to filter conditions in an SQL Server Database

From: Random and independent sampling of endogenous tryptic peptides from normal human EDTA plasma by liquid chromatography micro electrospray ionization and tandem mass spectrometry

Filter

Total correlations

Number of protein accessions

All

5,414,868

401,960

Filter 0

3,788,530

150,678

Filter 1

833,029

125,641

Filter 2

793,309

125,641

Filter 2

729,533

81,276

≥ 3 peptides

Per accession

  1. The results of 170 samples collected and maintained ice cold or preserved (82) versus samples incubated at room temperature for up to 72 h (88) prior to C18 solid phase extraction and micro LC–ESI–MS/MS with a linear ion trap are shown