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Table 2 The sum peptide-to-protein corrections per LC–ESI–MS/MS run from the MASCOT, OMSSA, X!TANDEM and SEQUEST algorithms over the experimental treatments of preserved and degraded human EDTA plasma

From: Random and independent sampling of endogenous tryptic peptides from normal human EDTA plasma by liquid chromatography micro electrospray ionization and tandem mass spectrometry

TreatmentName

Sum.Correlations

N

CorrelationsPerRun

FD-20 °C

346,290

12

28,858

− 80 °C

254,832

7

36,405

LN

401,209

11

36,474

FDRT

280,050

12

23,338

ICE_INHIB_1 h

42,197

2

21,099

ICE_INHIB_4 h

44,270

2

22,135

ICE_INHIB_8 h

90,814

4

22,704

ICE_INHIB_24 h

102,271

4

25,568

ICE_INHIB_48 h

80,709

4

20,177

ICE_INHIB_72 h

77,590

3

25,863

ICE_1 h

49,197

2

24,599

ICE_4 h

74,232

3

24,744

ICE_8 h

66,377

2

33,189

ICE_24 h

115,975

4

28,994

ICE_48 h

107,433

5

21,487

ICE_72 h

113,023

3

37,674

RT_1 h

538,873

12

44,906

RT_4 h

351,457

12

29,288

RT_8 h

327,895

10

32,790

RT_12 h

435,866

12

36,322

RT_24 h

507,283

12

42,274

RT_48 h

349,692

10

34,969

RT_72 h

403,881

11

36,716

RT_96 h

227,777

6

37,963

  1. The redundant correlations made by the sum of all four algorithms to any human protein are shown. Abbreviations: FD-20 °C, Freeze Dried and stored in an electric freezer at − 20 °C for 1 year; − 80 °C, fresh plasma stored in an electric freezer at – 80 °C for 1 year; LN, fresh plasma stored in liquid nitrogen for 1 year; FDRT, freeze dried and stored at room temperature for 1 year; ICE_INHIB, fresh plasma stored on ice with a cocktail of protease inhibitors; ICE, fresh plasma stored on ice; RT, room temperature