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Table 2 The sum peptide-to-protein corrections per LC–ESI–MS/MS run from the MASCOT, OMSSA, X!TANDEM and SEQUEST algorithms over the experimental treatments of preserved and degraded human EDTA plasma

From: Random and independent sampling of endogenous tryptic peptides from normal human EDTA plasma by liquid chromatography micro electrospray ionization and tandem mass spectrometry

TreatmentName Sum.Correlations N CorrelationsPerRun
FD-20 °C 346,290 12 28,858
− 80 °C 254,832 7 36,405
LN 401,209 11 36,474
FDRT 280,050 12 23,338
ICE_INHIB_1 h 42,197 2 21,099
ICE_INHIB_4 h 44,270 2 22,135
ICE_INHIB_8 h 90,814 4 22,704
ICE_INHIB_24 h 102,271 4 25,568
ICE_INHIB_48 h 80,709 4 20,177
ICE_INHIB_72 h 77,590 3 25,863
ICE_1 h 49,197 2 24,599
ICE_4 h 74,232 3 24,744
ICE_8 h 66,377 2 33,189
ICE_24 h 115,975 4 28,994
ICE_48 h 107,433 5 21,487
ICE_72 h 113,023 3 37,674
RT_1 h 538,873 12 44,906
RT_4 h 351,457 12 29,288
RT_8 h 327,895 10 32,790
RT_12 h 435,866 12 36,322
RT_24 h 507,283 12 42,274
RT_48 h 349,692 10 34,969
RT_72 h 403,881 11 36,716
RT_96 h 227,777 6 37,963
  1. The redundant correlations made by the sum of all four algorithms to any human protein are shown. Abbreviations: FD-20 °C, Freeze Dried and stored in an electric freezer at − 20 °C for 1 year; − 80 °C, fresh plasma stored in an electric freezer at – 80 °C for 1 year; LN, fresh plasma stored in liquid nitrogen for 1 year; FDRT, freeze dried and stored at room temperature for 1 year; ICE_INHIB, fresh plasma stored on ice with a cocktail of protease inhibitors; ICE, fresh plasma stored on ice; RT, room temperature