Fig. 1

Sequence alignment of reviewed UniProtKB Ag85B protein sequences entries. Shaded amino acids indicate divergence from the consensus sequence of the Mycobacterium tuberculosis complex or the consensus sequence of all proteins with aligned sequence at that position. Sequences are segregated vertically into three groups: members of the mycobacterium tuberculosis complex (5), common pulmonary NTM (3), and other NTM causing human disease (3). Symbols indicate positions with complete conservation (*) or conservation between groups of amino acids with strongly (:) or weakly (.) similar properties. Lines above the sequence indicate observed and/or predicted tryptic peptides, with red font denoting the NDPTIQQK peptide and its variant extension (dashed symbols) in other species, and amino terminal quesiton marks (?) denoting peptide sequences where one or more species contains an amino terminal proline reside expected to suppress cleavage