Antigen 85B peptidomic analysis allows species-specific mycobacterial identification

Table 2 Predicted Ag85B peptide ions detectable by LC–MS/MS from mycobacteria commonly involved in pulmonary disease

Precursor ion (2+)	Peptide sequence	M. tb	M. av	M. in	M. ka
892.972	PGLPVEYLQVPSAAMGR	+
885.964	PGLPVEYLQVPSAGMGR		+	+
913.977	PGLPVEYLQVPSPSMGR				+
1068.048	VQFQSGGNNSPAVYLLDGLR	+
1039.537	VQFQSGGNGSPAVYLLDGLR		+	+
1068.540	VQFQSGGDNSPAVYLLDGLR				+
414.189	AGC[+ 57.0]QTYK	+
393.676	AGC[+ 57.0]STYK		+	+
400.684	AGC[+ 57.0]TTYK				+
1039.521	WETFLTSELPQWLSANR	+			+
993.496	WETFLTSELPSYLASNK		+
1014.010	WETFLTSELPQYLASNK			+
838.362	AADMWGPSSDPAWER	+
837.870	ADAMWGPSSDPAWQR		+	+
845.868	ASDMWGPSSDPAWQR				+
520.772	NDPTQQIPK	+
394.225	LVANNTR	+
899.963	NDPSLHIPELVGHNTR		+
854.952	NDPSLQIPALVGNNTR			+
895.463	NDPSLHIPELVANNTR				+
274.656	SSNLK	+	+	+	+
452.722	GDLQSSLGAG	+
642.315	PDLQGTLGASPGGGG		+
637.305	PDLQSALGASSGGGG			+
494.265	GDLQASLGAR				+

M. tb: M. tuberculosis; M. av: M. avium; M. in: M. intracellulare; M. ka: M. kansasii
[+ 57.0] indicates the m/z introduced by cysteine carbamidomethylation

ISSN: 1559-0275