Fig. 1

Identification of lectins interacting with purified CEA. a Schematic presentation of lectin microarray for CEA glycosylation analysis. Lectin 1 and 2 bind two different glycosylation patterns of CEA. b Design of the lectin microarray containing 56 lectins. c Representative lectin microarray binding patterns of six different CEA concentrations. d Four representative lectins bind different concentrations of CEA. The smaller diagrams show the overall changes with the increase of CEA concentration. The red points in the dashed box show expanded portions of the small diagrams. Bars represent the mean values with standard deviation (SD). CY3-BSA and CY5-BSA are positive controls. Printing buffer 1 and 8 serve as negative controls. The color bar represents corresponding signal-to-noise value