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Fig. 2 | Clinical Proteomics

Fig. 2

From: Activation peptide of the coagulation factor XIII (AP-F13A1) as a new biomarker for the screening of colorectal cancer

Fig. 2

Skyline MS/MS filtering. a Skyline peptide tree for peptide AVPPNNSNAAEDDLPTVELQGVVPR with two (++) or three (+++) charges showing the four most intense extracted MS/MS fragments for light (1301.6590; 868.1084) and heavy precursors (1313.6803; 868.1084). b Skyline library of MS/MS spectra for the targeted peptides highlighting in blue matching fragments for an injected replicate. ★ highlight the four most intense extracted MS/MS fragments selected for the absolute quantification of AVPPNNSNAAEDDLPTVELQGVVPR c Chromatograms and peak intensity traces for three technical acquisitions of control patient (number accession P10) with heavy peptide spiked at 50 fmol/μL (130 ng/mL). d Peak area replicates normalized to the heavy peptide for the two (++) and three (+++) charged peptides (peak area light peptide/peak area heavy peptide)

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Clinical Proteomics

ISSN: 1559-0275