Comprehensive proteome analysis of nasal lavage samples after controlled exposure to welding nanoparticles shows an induced acute phase and a nuclear receptor, LXR/RXR, activation that influence the status of the extracellular matrix

Table 3 Absolute quantification of individual sample

Protein	Mean slope in NL	Mean slope in MilliQ water	LOD (fmol/µL)	Exposure	Mean; median; (max–min) concentration (fmol/µL)			Statistical evaluation
Protein	Mean slope in NL	Mean slope in MilliQ water	LOD (fmol/µL)	Exposure	Before exposure	After exposure	Day after exposure	Beta-value	95% CI	p value
MMP9	0.291	0.275	0.043	A	0.26; 0.14; (0.02–0.61)	0.14; 0.13; (0.02–0.37)	0.19; 0.13; (0.02–0.36)	0.77	0.63–0.93	0.015
MMP9	0.291	0.275	0.043	B	0.16; 0.15; (0.02–0.42)	0.16; 0.11 (0.03–0.52)	0.18; 0.16; (0.08–0.4)
A1AT	0.153	0.158	0.205	A	0.51; 0.37; (0.23–1.28)	0.50; 0.37; (0.23–1.6)	0.44; 0.52; (0.14–0.94)	1.20	0.77–1.87	0.369
A1AT	0.153	0.158	0.205	B	0.67; 0.51; (0.3–2.12)	0.52; 0.41; (0.21–1.84)	0.56; 0.45; (0.2–1.48)
A2 MG	0.184	0.153	0.075	A	0.52; 0.43; (0.09–0.13)	0.42; 0.20; (0.08–1.32)	0.35; 0.29; (0.05–0.95)	0.95	0.73–1.23	0.669
				B	0.49; 0.32; (0.16–1.13)	0.47; 0.24; (0.09–1.55)	0.48; 0.51; (0.11–1.44)
APOB	0.164	0.177	0.356	A/B	NA	NA	NA	–	–	–
Elastin	0.432*	0.339	0.339	A/B	NA	NA	NA	–	–	–

Mean slope of the calibration curve for each quantitative protein and the calculated LOD (determined from blank samples)
NL nasal lavage, A welding fume particle exposure, B filtered air exposure, NA no quantitative data could be obtained
* Big variation between the slopes

ISSN: 1559-0275