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Fig. 3 | Clinical Proteomics

Fig. 3

From: iTRAQ-based analysis of sperm proteome from normozoospermic men achieving the rescue-ICSI pregnancy after the IVF failure

Fig. 3

Experimental workflow of iTRAQ-based sperm proteome associated with different IVF outcomes. Equal amounts of sperm proteins from 20 normozoospermic men from IVF pregnancy group and 20 normozoospermic men from R-ICSI pregnancy group were pooled, digested by trypsin, and labelled by iTRAQ labels. After the labeled samples were separated by a pH10.0 reversed phase chromatography and a pH3.0 reversed phase chromatography, the fractions were spotted on the MALDI plates, and sequenced by the 5800 MALDI-TOF/TOF mass spectrometry. The identification and quantification of sperm proteins were performed by the software Protein pilot (version 4.0)

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Clinical Proteomics

ISSN: 1559-0275