Fig. 1
From: Proteomic profiling of liver tissue from the mdx-4cv mouse model of Duchenne muscular dystrophy
Overview of the complex multi-system pathophysiology of dystrophinopathy and proteomic workflow to analyse the mdx-4cv liver. Duchenne muscular dystrophy is caused by primary abnormalities in dystrophin and triggers progressive skeletal muscle wasting, cardio-respiratory abnormalities and cognitive impairments. In addition, X-linked muscular dystrophy is also characterized by secondary effects on a variety of organ systems including the liver. Proteome-wide changes in liver tissue were determined by comparative proteomics using the dystrophic mdx-4cv mouse model of Duchenne muscular dystrophy. Results obtained by mass spectrometry using an Orbitrap Fusion Tribrid apparatus were analysed by systems bioinformatics, and key findings were confirmed by verification studies employing immunoblotting and immunofluorescence microscopy