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Table 1 Characteristics of 33 antigens incorporated into the expression library vaccines

From: Identification of Cytauxzoon felis antigens via protein microarray and assessment of expression library immunization against cytauxzoonosis

Candidate #~ Location in C. felis genome! Selection criteria for microarray inclusion Selection criteria for vaccine inclusion Orthologue and/or synteny% Signal peptide (Y/N) Transmembrane domains Antigenic sites
1 contig00088:95434-96586(−) Antigenic features Differential reactivity Orthologous to conserved hypothetical protein (T. equi BEWA_039780) Y 1 13
2 contig00034:39570-41574(+) Antigenic features Differential reactivity None identified Y 1 34
3 contig00195:8035-8711(+)* Carboxy terminus of cf76: previously demonstrated antigenicity Differential reactivity Syntenic to conserved antigenic protein (T. parva p67, T. annulata SPAG-1, B. bovis BOV57) Y 0 N/A
4 contig00109:22439-22690(+) Antigenic features Differential reactivity None identified Y 1 46
5 contig00259:2101-4206(−) Antigenic features Differential reactivity Orthologous to conserved signal-peptide containing protein (T. equi BEWA_049350) Y 0 24
6 contig00010:254-1016(+) Antigenic features Differential reactivity None identified Y 1 11
7 contig00147:58097-59225(−) Antigenic features Differential reactivity None identified Y 1 11
8 contig00411:10993-12107(+)^ Antigenic features Differential reactivity None identified Y 1 13
9 contig00029:68709-70041(+) Antigenic features Differential reactivity None identified N 1 17
10 contig00237:32067-33665(+) Heterologous P. falciparum reactivity, previously demonstrated antigenicity Differential reactivity Orthologous to conserved hypothetical protein (T. equi BEWA_027410, P. falciparum PF3D7_0522400) N 0 N/A
11 contig00086:6446-7270(−) Antigenic features Differential reactivity None identified Y 0 12
12 contig00046:25575-26421(−) Antigenic features Differential reactivity None identified Y 1 10
13 contig00119:20915-22637(−) Antigenic features Differential reactivity Orthologous to conserved hypothetical protein (T. orientalis TOT_020000805) Y 9 14
14 contig00260:69016-71962(+) Antigenic features Differential reactivity Orthologous to conserved hypothetical protein (T. equi BEWA_039370) N 11 35
15 contig00052:839-1019(+) Antigenic features Differential reactivity None identified Y 1 2
16 contig00071:75760-77446(+) Antigenic features Differential reactivity Orthologous to conserved hypothetical protein (T. orientalis TOT_040000434) Y 9 20
17 contig00006:15286-16508(+) Antigenic features Differential reactivity Orthologous to Theileria Sphingomyelin/lysocholinephospholipid- phospholipase C (T. equi BEWA_044200) Y 0 19
18 contig00156:32608..32875(−) Antigenic features Differential reactivity Orthologous to Babesia/Theileria/Plasmodium protein kinase domain containing protein, PK4 (T. equi BEWA_042970, P. falciparum PF3D7_0628200) N 2 46
19 contig00195:6540-8711(+)* Full length cf76: previously demonstrated antigenicity Differential reactivity Syntenic to conserved antigenic protein (T. parva p67, T. annulata SPAG-1, B. bovis BOV57) Y 0 N/A
20 contig00214:29581-33385(+) Antigenic features Differential reactivity Orthologous to Theileria/Babesia p-type ATPase (T. equi BEWA_037450) N 8 52
21 ccontig00047:67240-68739(+)+ Antigenic features Differential reactivity Orthologous to conserved hypothetical protein (T. orientalis TOT_010000130) Y 1 49
22 contig00093:7841..7964(+) Orthology to Theileria antigenic protein (TaSE) Differential reactivity Orthologous to conserved antigenic protein (TaSE, Schizont Protein E, B. bovis secretory protein) N 0 20
23 contig00130:22711..24075(−) Antigenic features Differential reactivity Orthologous to conserved putative cysteine protease (T. equi BEWA_018680) N 1 16
24 contig00137:10059-13245(−) Antigenic features Differential reactivity Orthologous to conserved hypothetical protein (T. equi BEWA_041510) Y 11 37
25 contig00088:68222-69173(−) Antigenic features Differential reactivity Orthologous to conserved hypothetical protein (T. equi BEWA_011550) N 5 9
26 contig00232:10901..11056(−) Antigenic features Differential reactivity Orthologous to putative 50S ribosomal protein L17e (T. equi BEWA_024510) Y 0 9
27 contig00433:34958-36380(+) Antigenic features Differential reactivity Orthologous to conserved hypothetical protein (T. equi BEWA_053510) Y 11 14
28 contig00147:21973-23388(+) Antigenic features Differential reactivity Orthologous to conserved hypothetical protein (T. equi BEWA_041510) Y 9 16
29 contig00145:9-1059(−) Antigenic features Differential reactivity None identified Y 0 17
30 contig00167:23417-24638(−) Orthology to Theileria antigenic protein (TaSP, PIM) Differential reactivity Orthologous to conserved antigenic protein (TaSP, PIM) N 3 7
31 contig00063:31158-31905(−) Heterologous P. falciparum reactivity, orthology to Elongation Factor-1 Cross-reactive with near differential reactivity, previously studied in separate experiment Orthologous to Elongation factor-1 N 0 N/A
32 contig00062:98497-98689(+) Antigenic features Increased reactivity in acute serum None identified N 9 14
33 contig00079:101222-102015(+) Antigenic features Increased reactivity in acute serum Orthologous to conserved hypothetical protein (T. equi BEWA_022300) N 3 12
  1. ~Candidates 1–30 were assigned numbers based on descending average seroreactivity to infected feline serum. Candidate 31–33 were chosen for vaccine inclusion for other reasons and randomly assigned numbers
  2. !Location in C. felis genome refers to contig coordinates on PiroplasmaDB. Positive or negative sense are indicated in parenthesis
  3. *Candidate 19 = Full length cf76, Candidate 3 = C-terminal region of cf76. In addition to being in CF-Library, these two candidate comprise CF-1
  4. ^Candidate 8 = Not included in CF-Library (unable to amplify)
  5. +Candidate 21 = One portion of a larger ORF; indicated as “contig00047:66184-70054e2s1” in Additional file 1: Supplementary Data 1
  6. %Gene ID of top orthologues (highest score/E-value) to newly identified C. felis antigens and/or C. felis antigens selected due to heterologous P. falciparum reactivity are listed in parentheses as pertinent. Gene ID listed refers to PiroplasmaDB or PlasmoDB nomenclature
  7. N/A Not assessed