Fig. 3

Comparison between DIA spectral libraries. The analysis of the vDIA data was carried out with three spectral libraries: a project specific, a urine reference library appended in Spectronaut and the pan human library. a Basic information of the spectral libraries: the number of proteins and peptides covered by the libraries and the MS instrument used for library generation. b Overlap of identified proteins depending on the spectral libraries. c, d Total number of identified proteins and peptides were plotted. The column is separated into proteins or peptides which were detected in common (dark blue) and those detected in only one or two of three replicates (gray). e, f Distribution of quantified proteins and peptides in each CV% levels