Fig. 4

Performance of the urinary proteome profiling workflow. a Identification reproducibility of the entire workflow. b Correlation of the quantified protein intensities between replicate 1 and 3. Correlation of the protein intensities between replicate 1 and 2, and replicate 2 and 3 were appended in Additional file 4: Figure S1. c Quantification precision of the workflow. d Abundance distribution of the quantified proteins. Some disease-related biomarkers with known urinary concentration as reported form previous immunoassay screening are labeled [41]. GSTA2 Glutathione S-transferase A2, NC1 Collagen alpha-1(XVIII) chain, IBP-6 Insulin-like growth factor-binding protein 6, IL-1R-2 Interleukin-1 receptor type 2, Hsp60 60 kDa heat shock protein. e Top 20 of Gene Ontology enrichments for cellular component. f Comparative analysis of the urine and plasma proteome. The protein identifiers in different datasets were all converted to gene names for easier comparison