Fig. 1

Sample complement and workflow of experimental design to track the NHP proteomic response during infection with EBOV or Burkholderia pseudomallei. a NHP plasma samples used for this study including designations for the first day of detectable viremia or positive blood culture as well as outcome. b Sample processing workflow. SDS PAGE sample buffer and heat were used to inactivate pathogens in plasma samples serially collected from EBOV- or Bp-infected rhesus macaques. Filter-assisted sample prep (FASP) was employed to remove the buffer and perform reduction/alkylation, trypsin digestion and TMT labelling. After TMT labelling, serially collected samples from each NHP were mixed together allowing the simultaneous analysis of samples from 6 post-infection time points in a single LC–MS/MS run