Skip to main content

Advertisement

Table 3 Tryptic peptides and transitions used in MRM assay

From: Rapid detection of colistin resistance protein MCR-1 by LC–MS/MS

Peptide Charge Precursor (Da) T1 T2 T3 T4 T5 T6
DTFPQLAK 2+ 460.2478 y5 + (16.7) y6 ++ (16.7) y3 + (22.7) y6 + (22.7) y5 ++ (16.7)  
556.3453 Da 352.2105 Da 331.2340 Da 703.4137 Da 278.6763 Da  
SVPAFFWTDK 2+ 599.3006 y8 + (22) y6 + (28) y4 + (34) y7 + (31) y5 + (28) y3 + (34)
1011.4934 Da 843.4026 Da 549.2667 Da 914.4407 Da 696.3352 Da 363.1874 Da
ADHVSFNGYER 2+ 647.7942 y7 + (29.2) y8 + (29.2) y9 + (29.2) y6 + (29.2)   
872.3897 Da 971.4581 Da 1108.5170 Da 785.3577 Da   
  1. (): collision energy in parentheses