Table 4 rdotp and R ratio values for isolates used in assay development and mcr-1-containing isolates in validation set
From: Rapid detection of colistin resistance protein MCR-1 by LC–MS/MS
Assay | Group | Sample | rdotp value/R ratio value | ||
|---|---|---|---|---|---|
DTFPQLAK | SVPAFFWTDK | ADHVSFNGYER | |||
Method development | mcr-1 | P1 | 0.98/1.17 | 0.99/0.20 | 1.0/0.45 |
P2 | 0.99/0.95 | 0.98/0.19 | 1.0/0.34 | ||
P3 | 0.99/1.27 | 0.99/0.26 | 1.0/0.47 | ||
P4 | 0.99/1.13 | 0.99/0.22 | 1.0/0.38 | ||
P5 | 0.96/0.27 | 0.96/0.07 | 0.98/0.13 | ||
Negatives | N1 | 0.43/0.01 | 0.49/0.003 | 0.41/0.24 | |
N2 | 0.61/0.01 | 0.76/0.01 | 0.91/0.10 | ||
Method test | Blinded unknowns | U9 | 0.97/0.12 | 0.99/0.08 | 1.0/0.07* (0.9/0.09) |
U18 | 0.98/0.31 | 0.95/0.34* (0.76/0.5) | 0.99/0.20 | ||
U25 | 0.99/0.43 | 0.88/0.64* (0.71/0.9) | 0.99/0.32 | ||
U33 | 0.98/0.14 | 0.99/0.14 | 1.0/0.11* (0.75/0.18) | ||
U42 | 0.98/0.17 | 0.98/0.14 | 0.93/0.17* (0.74/0.27) | ||
U65 | 0.96/0.18 | 1.0/0.08 | 0.99/0.11* (0.91/0.14) | ||
U66 | 0.96/0.16 | 0.99/0.08 | 0.99/0.09* (0.79/0.15) | ||
U74 | 0.98/0.13 | 1.0/0.1 | 0.99/0.07* (0.76/0.12) | ||
U100 | 0.98/0.22 | 0.93/0.21* (0.74/0.32) | 1.0/0.09 | ||