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Table 4 rdotp and R ratio values for isolates used in assay development and mcr-1-containing isolates in validation set

From: Rapid detection of colistin resistance protein MCR-1 by LC–MS/MS

Assay Group Sample rdotp value/R ratio value
DTFPQLAK SVPAFFWTDK ADHVSFNGYER
Method development mcr-1 P1 0.98/1.17 0.99/0.20 1.0/0.45
P2 0.99/0.95 0.98/0.19 1.0/0.34
P3 0.99/1.27 0.99/0.26 1.0/0.47
P4 0.99/1.13 0.99/0.22 1.0/0.38
P5 0.96/0.27 0.96/0.07 0.98/0.13
Negatives N1 0.43/0.01 0.49/0.003 0.41/0.24
N2 0.61/0.01 0.76/0.01 0.91/0.10
Method test Blinded unknowns U9 0.97/0.12 0.99/0.08 1.0/0.07* (0.9/0.09)
U18 0.98/0.31 0.95/0.34* (0.76/0.5) 0.99/0.20
U25 0.99/0.43 0.88/0.64* (0.71/0.9) 0.99/0.32
U33 0.98/0.14 0.99/0.14 1.0/0.11* (0.75/0.18)
U42 0.98/0.17 0.98/0.14 0.93/0.17* (0.74/0.27)
U65 0.96/0.18 1.0/0.08 0.99/0.11* (0.91/0.14)
U66 0.96/0.16 0.99/0.08 0.99/0.09* (0.79/0.15)
U74 0.98/0.13 1.0/0.1 0.99/0.07* (0.76/0.12)
U100 0.98/0.22 0.93/0.21* (0.74/0.32) 1.0/0.09
  1. *After removal of one transition. The numbers in paranthese () are unadjusted values