Skip to main content

Table 2 Analytical performance of the 7-plex immunoassay

From: A multiplex immunoassay of serum biomarkers for the detection of uveal melanoma

 

Mean (pg/mL)

Intra-assay Precision (%CV)

Inter-assay Precision (%CV)

LOB (pg/mL)

LLOQ (pg/mL)

ULOQ (pg/mL)

Triplicates* (%CV)

 

QC1

QC2

QC1

QC2

QC1

QC2

    

OPN

13650.0

1435.0

4.4

6.8

5.2

14.5

278.8

389.5

40024.5

7.1

MIA

670.0

67.5

2.3

3.9

7.0

14.2

12.5

16.3

1999.0

4.7

CEACAM-1

9540.0

967.5

7.5

7.4

5.4

20.8

351.2

548.5

30079.5

5.0

MIC-1

2977.5

297.5

3.0

3.6

3.5

5.0

3.0

68.5

7407.0

3.5

SPON1

7825.0

762.5

2.9

2.9

2.8

4.5

50.8

185.3

24145.0

3.2

POSTN

8100.0

710.0

2.9

4.0

3.4

10.2

146.1

186.3

25081.3

3.4

HSP27

637.5

62.5

3.0

2.5

3.2

8.0

5.3

16.0

2001.3

2.7

  1. QC1, high control. QC2, low control. LOB, limit of black. LLOQ, lower limit of quantitation. ULOQ, upper limit of quantification. *mean of  %CV for triplicates in all samples for each protein