Fig. 1
From: Deep undepleted human serum proteome profiling toward biomarker discovery for Alzheimer’s disease
Experimental scheme of deep undepleted serum proteome analysis using TMT–LC/LC–MS/MS. AD and control serum samples were extracted, digested, labeled and pooled. The pooled peptide mixture was resolved in a 3 h gradient by basic pH RPLC, with fractions collected every minute (n = 180). Each collected fraction was subjected to the analysis by automated nanoscale acid pH RPLC, coupled with high resolution tandem mass spectrometry. All resulting data were analyzed by the JUMP software suite