Fig. 6
From: Deep undepleted human serum proteome profiling toward biomarker discovery for Alzheimer’s disease
Validation of selected proteins by TOMAHAQ targeted LC-MS3 method a TOMAHAQ used synthetic trigger peptides which were spiked into a mixture of multiplexed samples. Monitoring trigger peptides enabled quantification of target peptides. MS3 analyses of the target peptides were based on pre-defined b or y ions from target MS2 spectra, and the resulting reporter ions were used for quantification. b Validation of known candidate proteins PCK2 and AK2