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Table 2 Differences in the glycopattern of serum proteins between adenocarcinoma groups and healthy control by the lectin microarray analysis based on data of 18 lectins giving significant differences

From: Stage-associated differences in the serum N- and O-glycan profiles of patients with non-small cell lung cancer

Lectin

Specificity

Fold change

I/II Adenocarcinoma/healthy

III Adenocarcinoma/healthy

IV Adenocarcinoma/healthy

Jacalin

Galβ1-3GalNAcα-Ser/Thr(T), sialyl-T(ST)

1.68

1.86***

2.11**

HHL

High-Mannose, Manα1-3Man, Manα1-6Man, Man5-GlcNAc2-Asn

2.85**

3.16*

3.13*

PTL-I

GalNAc, GalNAcα-1, 3Gal, GalNAcα-1, 3Galβ-1,3/4Glc

3.49**

3.85**

2.81*

SJA

Terminal in GalNAc and Gal

2.98**

2.73**

1.55

AAL

Fucα1-6GlcNAc(core fucose), Fucα1-3 (Galβ1-4) GlcNAc

2.09***

2.71**

3.57**

LTL

Fucα1-2Galβ1-4GlcNAc, Fucα1-3(Galβ1-4) GlcNAc

0***

0***

0**

GSL-I

αGalNAc,GalNAcα-Ser/Thr (Tn) and αGal

/**

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/***

DBA

GalNAcα-Ser/Thr(Tn) and GalNAcα1-3Gal

4.27*

–

–

RCA-120

β-Gal, Galβ-1, 4GlcNAc(type II), Galβ1-3GlcNAc (type I)

0.13**

0.10***

0.09**

STL

Trimers and tetramers of GlcNAc, core (GlcNAc) of N-glycan, oligosaccharide containing GlcNAc and MurNAc

0.43**

0.45**

0.44**

BS-I

αGal and αGalNAc

/***

/***

/**

ConA

High-Mannose, Manα1-6(Manα1-3)Man, terminal GlcNAc

0.35***

0.35***

0.36***

DSA

β-D-GlcNAc, (GlcNAcβ1-4)n, Galβ1-4GlcNAc

3.77***

3.62***

3.49***

SBA

Terminal GalNAc (especially GalNAcα1-3Gal)

11.75***

13.96***

16.31***

PSA

α-D-Man, Fucα-1, 6GlcNAc (core fucose), α-D-Glc

9.36**

5.92***

6.20**

UEA-I

Fucα1-2Galβ1-4GlcNAc

7.99

5.29***

4.46**

MAL-I

Galβ-1,4GlcNAc, Siaα2-3Gal, Galβ1-3GlcNAc, Siaα2-3

/**

/***

/***

BPL

Galβ1-3GalNAc, terminal GalNAc

/**

/*

/*

  1. –, no significant difference; /, the denominator of the fold change was zero
  2. *P < 0.05, **P < 0.01, ***P < 0.001