Fig. 1

Differentially expressed proteins in the longevous and normal groups. TMT-based proteomic technique was used to study the protein characteristics in longevity families. Differentially expressed proteins and were defined as having fold changes > 1.2 (> 1.20 increased or < 0.83 decreased). A two tail Student’s T-test was performed and a p-value < 0.05 was considered to be statistically significant. a The volcano plot shows the up- (red) or down-regulated (green) proteins between the two groups. b Hierarchical clustering of differentially expressed proteins. The color scale bar locates in the bottom, and green and red indicate decreased and increased levels of the identified poteins, respectively. A1, A2 and A3, 3 replicates of the longevous group; B1, B2 and B3, 3 replicates of the normal group. c Biological processes, d cellular components and e molecular functions of GO annotation based function classification of the differentially expressed proteins