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Table 3 Identified proteins (autoantigens) differentially recognized by plasma autoantibodies of the longevous and normal groups, using human proteome microarrays

From: Plasma proteomic and autoantibody profiles reveal the proteomic characteristics involved in longevity families in Bama, China

No.

Name

Ratioa

p-valuea

Protein description

Subcellular location

1b

CASC4

1.10

0.026

Cancer susceptibility candidate 4

Cytoplasm

2b

DPY30

1.28

0.029

Histone methyltransferase complex regulatory subunit

Nucleus

3b

PMVK

1.18

0.044

Phosphomevalonate kinase

Cytoplasm

4b

HSPA2

1.23

0.014

Heat shock protein family A (Hsp70) member 2

Nucleus

5b

IFIT3

1.40

0.001

Interferon induced protein with tetratricopeptide repeats 3

Cytoplasm

6b

SCRN2

1.29

0.032

Secernin 2

Cytoplasm

7c

ZNF207

0.56

0.048

Zinc finger protein 207

Nucleus

8d

ARL2BP

1.38

0.030

ADP ribosylation factor like GTPase 2 binding protein

Extracellular

9d

GCK

1.24

0.031

Glucokinase

Cytoplasm

10d

ING3

1.36

0.012

Inhibitor of growth family member 3

Nucleus

11d

NXPH2

1.29

0.006

Neurexophilin 2

Cytoplasm

12d

RUVBL2

1.22

0.023

RuvB like AAA ATPase 2

Cytoplasm

13d

TPD52L1

1.84

0.005

Tumor protein D52-like 1

Extracellular

14d

ORMDL2

0.77

0.020

ORMDL sphingolipid biosynthesis regulator 2

Cytoplasm

  1. aHuman proteome microarrays were used to study the autoantibody characteristics in longevity families. A protein recognized in all three samples in one group and not recognized by any sample in another group was defined as a specifically autoantigen that represented a specifically expressed autoantibody. And an antibody with its antigen–antibody reactive signal intensity of featuring a fold change (Longevous/Normal ratio) of > 1.2 (> 1.20 increased or < 0.83 decreased) between the two groups was regarded as a differentially expressed autoantibody. A two tail Student’s T-test was performed and a p-value < 0.05 was considered to be statistically significant
  2. bAutoantigens specifically reacted with IgG in the longevous group (autoantigens reacted with all 3 samples in the longevous group and not reacted with any samples in the normal group)
  3. cAutoantigens specifically reacted with IgG in the normal group (autoantigens reacted with all 3 samples in the normal group and not reacted with any samples in the longevous group)
  4. dDifferential autoantibodies (presented as autoantigens) that had a fold change between the two groups of > 1.2 or < 0.83 and a p value of < 0.05