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Fig. 8 | Clinical Proteomics

Fig. 8

From: Screening differentially expressed proteins from co-cultured hematopoietic cells and bone marrow-derived stromal cells by quantitative proteomics (SILAC) method

Fig. 8Fig. 8

Western blotting of differentially expressed proteins. Selected proteins of CKAP4, CD44, LCP1 and CAPG from SILAC dataset. a, b The scheme of different co-culture model (c). Protein expression of CKAP4, CD44, LCP1 and CAPG to confirm SILAC results in different co-cultured models. GAPDH served as loading control. d, e “KG1a(HS5)”: KG1a after co-culture with HS5, “KG1a(HS27a)”: KG1a after co-culture with HS27a, “SKM-1(HS27a)”: SKM-1 after co-culture with HS27a, “SKM-1(HS5)”: SKM-1 after co-culture with HS5,”HS5(KG1a)”: HS5 after co-culture with KG1a. “HS27a(KG1a)”: HS27a after co-culture with KG1a, “HS27a(SKM-1)”: HS27a after co-culture with SKM-1, “HS5(SKM-1)”: HS5 after co-culture with SKM-1. f Flow cytometry analysis confirmed the proliferation of KG1a after co-cultured with HS27a. g Western blotting to verify the efficiency of knockdown CD44 in KG1a. h Detects Cell proliferation was detected by Cell Counting Kit-8 (CCK-8 kit). ScrRNA, KG1a is interfered by Scramble RNA. SiRNA#1, siRNA#2, siRNA#3 are three different specific siRNAs used to knock down CD44

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