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Fig. 2 | Clinical Proteomics

Fig. 2

From: Clinical method evaluation of hemoglobin S and C identification by top-down selected reaction monitoring and electron transfer dissociation

Fig. 2

Results of CEX-HPLC method and corresponding TD SRM ETD method for two samples are presented. a Results of heterozygote Hb S sample. On top, results table and chromatogram are presented. Hb A is detected at 52%, 1.734 min of RT and Hb S is detected at 36.8%, 3.439 min of RT. The corresponding peaks in the chromatogram are highlighted by a red box. On bottom, TD SRM ETD results are presented in a table for Hb A, Hb S, Hb C and Hb E chains ratio and color code results. If the targeted Hb is present, the corresponding box is green. Hb A and S are detected with a ratio of 63% and 29% respectively (green boxes). b Results of homozygote Hb S sample. On top, results table and chromatogram are presented. Hb A is detected at 1.4%, 1.773 min of RT and Hb S is detected at 83.9%, 3.380 min of RT. The corresponding peaks in the chromatogram are highlighted by a red box. On bottom, TD SRM ETD results are presented in a table for Hb A, Hb S, Hb C and Hb E chains ratio and color code result. If the targeted Hb is present, the corresponding box is green. Only Hb S is detected with a ratio of 93% (green box)

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Clinical Proteomics

ISSN: 1559-0275