Clinical method evaluation of hemoglobin S and C identification by top-down selected reaction monitoring and electron transfer dissociation

Table 2 Isolation parameters for α chain, Hb A and Hb S β chains. Hb C, Hb E, Hb D-Punjab and Hb O-Arab isolation is based on the same parameters as Hb A β chain

Chain	Charge state	First isolation step		Second isolation step
Chain	Charge state	Precursor ion (m/z)	Isolation width (m/z)	Precursor ion (m/z)	Isolation width (m/z)
β^S	[M+19H]¹⁹⁺	837	11.5	835.3	2.5
β	[M+19H]¹⁹⁺	837	11.5	837	3
α	[M+18H]¹⁸⁺	841.9	12.5	841.9	2.5

Hb C, Hb E, Hb D-Punjab and Hb O-Arab isolation is based on the same parameters as Hb A β chain

ISSN: 1559-0275