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Table 2 Isolation parameters for α chain, Hb A and Hb S β chains. Hb C, Hb E, Hb D-Punjab and Hb O-Arab isolation is based on the same parameters as Hb A β chain

From: Clinical method evaluation of hemoglobin S and C identification by top-down selected reaction monitoring and electron transfer dissociation

ChainCharge stateFirst isolation stepSecond isolation step
Precursor ion
(m/z)
Isolation width
(m/z)
Precursor ion
(m/z)
Isolation width
(m/z)
βS[M+19H]19+83711.5835.32.5
β[M+19H]19+83711.58373
α[M+18H]18+841.912.5841.92.5
  1. Hb C, Hb E, Hb D-Punjab and Hb O-Arab isolation is based on the same parameters as Hb A β chain