TY - JOUR AU - Ward, Michael D. AU - Kenny, Tara AU - Bruggeman, Ernie AU - Kane, Christopher D. AU - Morrell, Courtney L. AU - Kane, Molly M. AU - Bixler, Sandra AU - Grady, Sarah L. AU - Quizon, Rachel S. AU - Astatke, Mekbib AU - Cazares, Lisa H. PY - 2020 DA - 2020/03/17 TI - Early detection of Ebola virus proteins in peripheral blood mononuclear cells from infected mice JO - Clinical Proteomics SP - 11 VL - 17 IS - 1 AB - Detection of viral ribo-nucleic acid (RNA) via real-time polymerase chain reaction (RT-PCR) is the gold standard for the detection of Ebola virus (EBOV) during acute infection. However, the earliest window for viral RNA detection in blood samples is 48–72 h post-onset of symptoms. Therefore, efforts to develop additional orthogonal assays using complementary immunological and serological technologies are still needed to provide simplified methodology for field diagnostics. Furthermore, unlike RT-PCR tests, immunoassays that target viral proteins and/or early host responses are less susceptible to sequence erosion due to viral genetic drift. Although virus is shed into the bloodstream from infected cells, the wide dynamic range of proteins in blood plasma makes this a difficult sample matrix for the detection of low-abundant viral proteins. We hypothesized that the isolation of peripheral blood mononuclear cells (PBMCs), which are the first cellular targets of the Ebola virus (EBOV), may provide an enriched source of viral proteins. SN - 1559-0275 UR - https://doi.org/10.1186/s12014-020-09273-y DO - 10.1186/s12014-020-09273-y ID - Ward2020 ER -