Fig. 1

Viral titers during EBOV infection in mice from two independent cohorts. RNA was extracted from whole blood and subjected to RT-PCR using primers specific for EBOV GP. Samples were combined from 5 mice/ PI time point and run in triplicate for each experimental trial. For quantitative assessments, the average of the triplicate GE per reaction were determined and multiplied by 800 to obtain GE/mL plasma. Standard curves were generated using synthetic RNA. Error bars represent standard deviation. The limit of detection for this assay is 1 × 102 GE/mL of plasma (dotted line)