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Fig. 4 | Clinical Proteomics

Fig. 4

From: Development of a novel method for the quantification of tyrosine 39 phosphorylated α- and β-synuclein in human cerebrospinal fluid

Fig. 4

Quantification of pY39 αβ-syn peptide in CSF samples from PD and control individuals. a The experimental strategy for pY39 αβ-syn peptide enrichment from PD and control CSFs using the two-step enrichment approach. CSF proteins were digested with Lys-C and trypsin followed by the two-step enrichment. To minimize experimental biases, heavy standard pY39 αβ-syn peptide was added before starting the experiment. The endogenous light and heavy standard pY39 αβ-syn peptides were monitored under PRM mode followed by quantification using Skyline software. b The abundances of pY39 αβ-syn peptide in PD and control CSFs. c The abundances of Y39 αβ-syn peptide in PD and control CSFs. d The relative abundance of pY39 to Y39 αβ-syn peptide in PD and control CSFs

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Clinical Proteomics

ISSN: 1559-0275