From: Recent advances in mass spectrometry based clinical proteomics: applications to cancer research
Liquid biopsy | Protein quantitation | MS model | Sample preparation | Clinical question | Proteins detected | Patient cohort | References |
---|---|---|---|---|---|---|---|
Plasma | Isotopic label | LTQ Orbitrap | Immunodepletion, filtration, SCX fractionation | Pancreatic cancer, pancreatitis and healthy control plasma | 1300 | 3 | [156] |
Label-free DDA | LTQ Orbitrap & 5500 Q-trap | PDX, N-glycopeptide enrichment, SRM validation in human sera | Ovarian cancer biomarker development | 906 | 224 | [175] | |
Label-free SWATH | 5600 TOF | Immunodepletion, SCX, SAX, RPF, size-exclusion chromatography | Early diagnosis of CRC | 427 | 100 | [159] | |
5600 TOF | N-glycopeptide enrichment, Off-gel fractionation | Five different cancer types and their matched controls | 1151 | 284 | [172] | ||
Serum | Label-free DDA | LTQ Orbitrap | PDX, N-glycopeptide enrichment, Targeted validation in human | PCa diagnosis | 775 | 8 | [173] |
QE HF | PDX, N-glycopeptide enrichment, PRM validation in human sera | High grade serious ovarian cancer biomarkers and longitudinal monitoring | 2200 | 20 | [87] | ||
iTRAQ | 5600 TOF | Immunodepletion, SWATH verification | Proteins leaving lung cancer tumours into pulmonary veins | 1000 | 50 | [158] | |
Urine | Label-free DDA | 5600 TOF | Gel fractionation, RPF, IEF | Characterization of the healthy urine proteome | 6085 | 24 | [176] |
QE | MW-filtration, SCX, PRM validation | Renal cell carcinoma prognostic biomarkers | 2589 | 115 | [183] | ||
LTQ | Gel fractionation | Identify novel therapeutic targets for Wilms tumour | 6520 | 49 | [184] | ||
QE | Gel fractionation | Profiling urine from lung cancer patients and other tumors | 7408 | 46 | [185] | ||
Post-DRE urine | iTRAQ | OV | Ultracentrifugation, RPF | Discovery of new biomarker for high Gleason PCa | 4710 | 18 | [191] |
Label-free DDA | QE | Ultracentrifugation | Characterizing EVs from EPS in urine from PCa and healthy patients | 877 | 24 | [189] | |
SRM | TSQ Vantage | MW filtration, TFE | Targeted proteomics identifies signatures for extracapsular prostate cancer | 232 | 74 | [188] | |
Qtrap5500 | FASP | Biomarker validation for early detection and stratification of PCa | 64 | 107 | [192] |