Fig. 1

Experimental workflow of iTRAQ-based HFF proteomic study of the normal and the overweight status women. Equal amounts of HFF proteins from 20 normal-weight women and 20 overweight status women were pooled, digested by trypsin, and labelled by iTRAQ labels. After the labeled samples were separated by a pH 10.0 and a pH 3.0 reversed phase chromatography, the resultant fractions were spotted on the MALDI plates. Then, the peptides were sequenced by the 5800 MALDI-TOF/TOF mass spectrometry, and the quantification of HFF proteins were performed by the software of Protein pilot (version 4.0)