Fig. 6
Representative western blot analyses of four altered HFF proteins and validation of the expression of upregulated protein WAP four-disulfide core domain protein 2 (WFDC2) by ELISA. a The expressions of WAP four-disulfide core domain protein 2 (WFDC2), lactotransferrin (LTF), Prostate-specific antigen (KLK3), Fibronectin (FN1), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were confirmed by western blot. Asterisk denoted a statistically significant difference between the two groups; a WFDC2 concentration in the follicular fluid samples of 25 normal weight women and 25 overweight status women. ROC curve of WFDC2 distinguished the normal weight and overweight status women