Table 1 Identification results based on conventional urine culture and 16S rRNA gene sequencing
From: Identification of pathogens from native urine samples by MALDI-TOF/TOF tandem mass spectrometry
N.o. | Urine culture identification | DNA concentration (ng/μL) | 16S rRNA sequencing results |
|---|---|---|---|
UR1 | Klebsiella pneumoniae ESBL | 3.15 | 100% Enterobacteriaceae |
UR2 | Klebsiella oxytoca | 9.14 | 97% Enterobacteriaceae |
UR3 | Klebsiella pneumoniae ESBL | 6.01 | 90% Enterobacteriaceae; 5.1% Granulicatella; 1.1% Anaerococcus |
UR4 | Proteus mirabilis | 6.96 | 97.7% Proteus; 1% Enterobacteriaceae |
UR5 | Enterococcus faecalis | 16.22 | 21.8% Pseudomonas; 13.7% Propionibacterium acnes; 11% Lactobacillus helveticus; 8.1% Adhaeribacter; 8% Acinetobacter; 5.9% Staphylococcus; 4.9% Stenotrophomonas; 3.8% Hydrogenophaga; 3.6% Erysipelotrichaceae; 3.1% Corynebacterium; 3.1% Cellulomonas; 2.3% Aerococcus; 2% Acidovorax; 1.9% Lachnospiraceae; 1.6% Sphingobium |
UR6 | Enterococcus faecalis | 4.93 | 51.4% Enterococcus; 46.5% Enterococcaceae |
UR7 | Enterobacter cloaceae ESBL | 1.84 | 98% Enterobacter; 0.9% Proteus |
UR8 | Citrobacter koseri | 15.94 | 57.5% Citrobacter koseri; 4.5% Bacteroides; 3.7% Dysgonomonas; 2.7% Bacteroides; 2.6% Rikenellaceae; 2.3% Parabacteroides; 2% Desulfovibrionaceae; 2% Lachnospiraceae; 2% Ruminococcaceae; 1.9% Enterobacteriaceae; 1.3% Ruminococcus; 1.3% Erysipelotrichaceae; 1.2% Enterococcus; 1.1% Clostridiales |
UR9 | Proteus mirabilis | 0.45 | 96.7% Proteus; 2.4% Enterobacteriaceae; 1.2% Prevotella |
UR10 | Proteus mirabilis | 3.66 | 97% Proteus; 1.3% Enterobacteriaceae |
UR11 | Escherichia coli; Proteus mirabilis ESBL | 9.39 | 93% Enterobacteriaceae; 3.5% Proteus |
UR12 | Proteus mirabilis | 1.73 | 99.2% Proteus |
UR13 | Enterobacter aerogenes | 1.08 | 75.4% Enterobacteriaceae; 14% Lactobacillus delbrueckii; 4.2% Kluyvera; 4% Enterobacter; 1% Lactobacillus helveticus |
UR14 | Enterobacter cloacae | 4.14 | 95.4% Enterobacteriaceae; 1.2% Clostridium perfringens; 1% Bifidobacterium pseudolongum |
UR15 | Enterobacter cloacae; Enterococcus faecalis; E coli; Proteus mirabilis | 15.17 | 86.9% Proteus; 7.2% Enterobacteriaceae; 2..2% Enterobacter; 1% Rhodospirillaceae |
UR16 | Escherichia coli; Klebsiella pneumoniae | 15..04 | 91.2% Enterobacteriaceae; 8.6% Klebsiella |