Fig. 2

EV characterisation. a nanoparticle tracking analysis of extracted CSF EVs (grey lines indicate individual sample particle counts, solid green line indicates mean count for each particle size). b Transmission electron micrograph of extracted EVs (obtained from a pooled control sample). c EV marker proteins and contaminants (denoted by gene symbol) identified within the proteomic dataset in healthy control (yellow) and ALS first-visit (red) samples. d Gene ontology cellular component overrepresentation analysis, using identifications from the CSF proteome resource as the background list, indicates significant enrichment of exosome, microvesicle and extracellular vesicle terms. ALS: amyotrophic lateral sclerosis; EV: extracellular vesicle; FDR: false discovery rate; CSF: cerebrospinal fluid; CD9—UniProt P21926; SDCBP—O00560; CD81—P60033; PDCD6IP—Q8WUM4; HSPA8—P11142; CD82—P27701; CD63—P08962; TSG101—Q99816; FLOT1—O75955; FLOT2—Q14254; HSP90AB1—P08238; LAMP1—P11279; LAMP2—P13473; ALB—P02768; APOA1—P02647; APOA2—P02652; APOB—P04114