Fig. 4

Peptide amounts at different trypsin digestion times. Peptide amounts (mean ratio light/heavy) of the tested peptides at 16 h compared to 24 h (a) and 30 h (b). Green dots represent peptides with < 20% change after 16 h of digestion and CV < 20% between replicates, yellow dots represent peptides with < 20% change after 16 h digestion, but with CV > 20% between replicates and red dots represent peptides with > 20% change after 16 h digestion. Protein short name and the four first amino acids in the peptide sequence is shown for all peptides failing this test. The two peptides AQLLVVGSPGPVPR and ELDLNSVLLK are not included in this plot due to very high values compared to the rest. Measured abundance change at all time points are available in Additional file 8: Fig. S2