Skip to main content

Table 1 Immunoaffinity assay temperature, freeze-thaw, and extract, stability

From: A robust multiplex immunoaffinity mass spectrometry assay (PromarkerD) for clinical prediction of diabetic kidney disease

  4 °C RT 24 h Freeze–thaw Freeze–thaw Extract
(N = 3) 24 h vs 1 h 24 h vs 1 h RT vs 4 °C 2 vs 1 3 vs 1 24 h, 4 °C, (N = 86),
        Freeze–thaw Freeze–thaw Autosampler
Protein Accuracy Precision (% CV) Accuracy Precision (% CV) Accuracy Precision (% CV) Accuracy Precision (% CV) Accuracy Precision (% CV) Precision (% CV)
APOA4 101.6 10.0 92.6 7.4 84.0 3.6 107.4 13.3 100.8 19.0 0.9
CD5L 118.4 8.6 103.8 10.1 95.4 14.5 106.7 5.7 106.1 14.8 8.5
IBP3 95.3 13.9 91.8 5.5 99.6 4.3 101.1 11.9 95.2 15.1 2.1
  1. Accuracy data are expressed as average percentage (N = 3) compared to control value equalling 100%. Precision data are the average % CV of the replicate comparisons