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Table 1 Immunoaffinity assay temperature, freeze-thaw, and extract, stability

From: A robust multiplex immunoaffinity mass spectrometry assay (PromarkerD) for clinical prediction of diabetic kidney disease

 

4 °C

RT

24 h

Freeze–thaw

Freeze–thaw

Extract

(N = 3)

24 h vs 1 h

24 h vs 1 h

RT vs 4 °C

2 vs 1

3 vs 1

24 h, 4 °C, (N = 86),

       

Freeze–thaw

Freeze–thaw

Autosampler

Protein

Accuracy

Precision (% CV)

Accuracy

Precision (% CV)

Accuracy

Precision (% CV)

Accuracy

Precision (% CV)

Accuracy

Precision (% CV)

Precision (% CV)

APOA4

101.6

10.0

92.6

7.4

84.0

3.6

107.4

13.3

100.8

19.0

0.9

CD5L

118.4

8.6

103.8

10.1

95.4

14.5

106.7

5.7

106.1

14.8

8.5

IBP3

95.3

13.9

91.8

5.5

99.6

4.3

101.1

11.9

95.2

15.1

2.1

  1. Accuracy data are expressed as average percentage (N = 3) compared to control value equalling 100%. Precision data are the average % CV of the replicate comparisons