Fig. 6From: Clinical proteomics for prostate cancer: understanding prostate cancer pathology and protein biomarkers for improved disease managementShotgun Proteomics Workflow. The above schematic outlines the typical workflow implemented for shotgun proteomics. The core steps for sample preparation include protein extraction and proteolytic digestion of protein material into peptides. Optional steps include protein/peptide fractionation for increased proteome coverage of complex samples. Peak quantification and database searching are the key bioinformatics steps required for peptide/protein identification and label-free quantificationBack to article page