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Fig. 1 | Clinical Proteomics

Fig. 1

From: Evaluation of the potential of Pap test fluid and cervical swabs to serve as clinical diagnostic biospecimens for the detection of ovarian cancer by mass spectrometry-based proteomics

Fig. 1

Work flow for the proteomic analysis of ovarian cancer biospecimens. a A liquid based Pap test, cervical swab (Merocel™) and snap frozen primary tumor tissue were collected from a patient with high grade serous ovarian cancer and proteins were prepared for mass spectrometry analysis as described in the Methods. b Proteins were digested with trypsin using an in-solution digest (tumor tissue extract) or FASP (Pap test fluid and swab proteins). c Peptides were HPLC fractionated and analyzed by mass spectrometry. d MS/MS data were searched against a forward and reverse sequence human Uniprot database using Sequest. Data was analyzed with Scaffold 4. Proteins were identified with at least 2 peptides at 99% probability and 0.2% decoy False Discovery Rate (FDR)

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Clinical Proteomics

ISSN: 1559-0275