Skip to main content
Fig. 5 | Clinical Proteomics

Fig. 5

From: Global proteome profiling of human livers upon ischemia/reperfusion treatment

Fig. 5

Validation of ischemic groups and control groups using western blotting and qPCR. a The mRNA expressions of up-regulated proteins, such as, AXL, AIF1, ORM1, NUCB2, HEPA, and ARHGAP9 of control and ischemia/reperfusion livers were verified using quantitative real-time analysis. The error bars represent the mean ± SD. Statistical significance was analyzed with unpaired Student's t-test (*p < 0.05, **p < 0.01, ***p < 0.001). b The mRNA expressions of down-regulated proteins, such as, ECM1, APOC1, S1OOA1O and ACSL4 of control and ischemia/reperfusion livers were verified using quantitative real-time analysis. The error bars represent the mean ± SD. Statistical significance was analyzed with unpaired Student's t-test (*p < 0.05, **p < 0.01, ***p < 0.001). c The mRNA expressions of five PPAR signaling pathway-related proteins Perlipin, PCK1, APOA5, SCD and ACS were detected. d Western blot analysis of AXL, PCK1, Perlipin, CRP, ECM1 and SCD expressions of control livers (C1, C2 and C3) and ischemia/reperfusion livers (S1, S2 and S3). e Representative western blot analysis of ECM1 and AXL protein levels in livers from mice subjected to sham treatment or ischaemia for 1 h followed by reperfusion for the indicated times

Back to article page