Fig. 3

Quantification of down-regulation (0.56 ± 0.07) of intact N-glycopeptide INSSVK_N2H9F0S0 from N-glycoprotein RalBP1-associated Eps domain-containing protein 1 (Q96D71, N-glycosite N498) in MCF-7/ADR CSCs relative to MCF-7/ADR cells. (a–c) the isotopic envelope fingerprinting maps of the precursor ions in the three technical replicates; d selective fragmentation and the graphical fragmentation map of N-glycan moiety with the peptide backbone, e fragmentation and the graphical fragmentation map of the peptide backbone with one core GlucNAc, and f the annotated MS/MS spectrum with the matched fragment ions in representative spectrum 19,803 of TR1