Fig. 4

Quantification of up-regulation of (3.54 ± 0.33) of intact N-glycopeptide DAVNNITAK_N2H8F0S0 from N-glycoprotein Voltage-dependent calcium channel subunit alpha-2/delta-1 (P54289, N-glycosite N324) in MCF-7/ADR CSCs relative to MCF-7/ADR cells. (a– c) the isotopic envelope fingerprinting maps of the precursor ions in the three technical replicates; d selective fragmentation and the graphical fragmentation map of N-glycan moiety with the peptide backbone, e fragmentation and the graphical fragmentation map of the peptide backbone with one core GlucNAc, and f the annotated MS/MS spectrum with the matched fragment ions in representative spectrum 17,216 of TR1